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KMID : 0391119950040010050
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Ulsan University Medical Journal
1995 Volume.4 No. 1 p.50 ~ p.57
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Effects of growth factors on rabbit corneal endothelial cell culture
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Abstract
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In order to investigate the effects of epidermal growth factor (EGF), fibroblast growth factor(FGF) and transforming growth factor)β-(EGF-β), rabbit corneal endothelial cells were cultured. After adding growth factors individually or in
combination,
DNA synthesis of corneal endothelial cells was measured. EGF and FGF increased DNA synthesis of corneal endothelial cells at all concentration tested(0.03ng/ml - 300ng/ml) compared with the control.
The maximum increase of DNA synthesis was shown at the concentration of 0.3-10ng/ml of EGF or 10-30ng/ml of FGF. TGF-β reduced DNA synthesis of corneal endothelial cells at all concentration tested (0.03ng/ml-10ng/ml). DNA synthesis of corneal
endothelial cells was more increased when EGF and FGF were added in combination. TGF-β inhibited DNA synthesis induced by EGF or FGF when added at the same tie. These results indicate that combination of EGF and FGF has maximum effet in DNA
synthesis
increase of rabbit corneal endothelial cells.
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KEYWORD
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